Quantitative RT-PCR to Evaluate In Vivo Expression of Multiple Transgenes Using a Common Intron
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منابع مشابه
Quantitative RT-PCR to evaluate in vivo expression of multiple transgenes using a common intron.
An assay measuring RNA expression levels of a gene-encoded therapeutic must distinguish between endogenous mRNA and mRNA transcribed from the transgene. Specificity for the delivered transgene is especially critical when the treatment involves genes that are expressed in the target tissue. To facilitate uniform detection of transgene RNA without interference from endogenous mRNA, we have engine...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99273rr04